2019-nCoV Neutralizing Antibody Test (QDIC)
Cikakken Bayani:
Gwajin Innovita® 2019-nCoV IgM/IgG an yi niyya ne don gano ƙididdiga na neutralizing antibody zuwa novel coronavirus (2019-nCoV) a cikin jini na ɗan adam, plasma ko duka jini (jinin ɗan yatsa ko venous dukan jini) samfurori.
2019-nCoV ya ƙunshi manyan sunadaran tsari guda huɗu: furotin S, furotin E, furotin M da furotin N.Yankin RBD na furotin S na iya ɗaure ga mai karɓar saman sel ACE2.Nazarin ya nuna cewa samfuran mutanen da suka murmure daga kamuwa da cutar sankara na coronavirus suna da inganci don kawar da antibody.Za'a iya amfani da gano maganin antibody don tantance hasashen kamuwa da kwayar cuta da kuma kimanta tasirin bayan allurar.
Ka'ida:
Kit ɗin shine ƙididdigar ƙididdiga ta ƙididdige immunofluorescence chromatography don gano 2019-nCoV RBD ƙayyadaddun ƙwayoyin rigakafi na IgG a cikin jini na ɗan adam, plasma ko duka jini (jinin yatsa da jini gaba ɗaya) samfurori.Bayan da aka yi amfani da samfurin ga samfurin da kyau, idan ƙaddamar da ƙwayoyin rigakafi ya fi mafi ƙanƙanta iyakar ganowa, RBD takamaiman ƙwayoyin rigakafi na IgG za su amsa tare da sashi ko duk RBD antigen da aka yi wa lakabi da ƙananan microspheres na adadi don samar da fili na rigakafi.Sa'an nan kuma fili na rigakafi zai yi hijira tare da nitrocellulose membrane.Lokacin da suka isa yankin gwajin (T line), fili zai amsa tare da linzamin kwamfuta anti-human IgG (γ sarkar) mai rufi a kan nitrocellulose membrane kuma samar da wani kyalli line.Karanta ƙimar siginar mai walƙiya tare da mai nazarin immunoassay na fluorescence.Ƙimar siginar tayi daidai da abun ciki na neutralizing kwayoyin cuta a cikin samfurin.
Ko samfurin ya ƙunshi takamaiman ƙwayoyin rigakafi na RBD ko a'a, layin sarrafawa yakamata koyaushe ya bayyana a cikin taga sakamakon idan an yi aikin gwajin da kyau kuma reagent yana aiki kamar yadda aka yi niyya.Lokacin da kajin IgY antibody da aka yiwa lakabi da quantum dot microspheres yayi ƙaura zuwa layin sarrafawa (layin C), goat anti-chicken IgY antibody wanda aka riga aka rigaya akan layin C zai kama shi, kuma an samar da layin mai kyalli.Ana amfani da layin sarrafawa (layin C) azaman kulawar tsari.
Abun ciki:
| Abun ciki | Adadin | Ƙayyadaddun bayanai |
| IFU | 1 | / |
| Gwada kaset | 20 | Kowace jakar da aka hatimi mai ɗauke da na'urar gwaji guda ɗaya da na'urar bushewa ɗaya |
| Siffofin sinadarai | 3ml*1 gwangwani | 20mM PBS, Sodium Casein, ProClin 300 |
| Micropipette | 20 | Micropipette tare da layin alamar 20 μL |
| Lancet | 20 | / |
| Tashin barasa | 20 | / |
Tsarin Gwaji:
● Tarin Jini na Hantsi
● Karanta sakamakon tare da na'urar nazarin haske
