Rotavirus/Adenovirus/Norovirus Ag Gwajin
Amfani da Niyya
An yi nufin kit ɗin ne don gano kai tsaye da ƙwarewar ƙungiyar A rotavirus antigens, adenovirus antigens 40 da 41, norovirus (GI) da norovirus (GII) antigens a cikin samfuran feces na ɗan adam.
Kyakkyawan sakamakon gwaji yana buƙatar ƙarin tabbaci.Sakamakon gwaji mara kyau baya kawar da yiwuwar kamuwa da cuta.
Sakamakon gwajin wannan kit ɗin don nunin asibiti ne kawai.Ana ba da shawarar yin nazari mai zurfi game da yanayin dangane da bayyanar cututtuka na marasa lafiya da sauran gwaje-gwajen dakin gwaje-gwaje.
Takaitawa
Rotavirus (RV)wata muhimmiyar cuta ce da ke haifar da gudawa da ciwon ciki ga jarirai da kananan yara a duniya.Kololuwar abin da ke faruwa a cikin kaka, wanda kuma aka sani da "cututtukan kaka na jarirai da kananan yara".Yawan kamuwa da cututtukan cututtuka a cikin jarirai a cikin watanni da shekaru 2 ya kai kashi 62%, kuma lokacin shiryawa shine kwanaki 1 zuwa 7, gabaɗaya ƙasa da sa'o'i 48, yana bayyana ta hanyar matsananciyar gudawa da bushewa.Bayan ya mamaye jikin mutum, yana yin kwafi a cikin sel epithelial na ƙananan hanji kuma ana fitar da shi da yawa da najasa.
Adenovirus (ADV)kwayar halittar DNA ce mai dunkule guda biyu tare da diamita na 70-90nm.Kwayar cuta ce mai kama da icosahedral ba tare da ambulaf ba.Kwayoyin ƙwayoyin cuta sun ƙunshi bawoyin furotin da ainihin DNA mai ɗaure biyu.Enteric adenovirus nau'in 40 da nau'in 41 na rukunin rukunin F sune mahimman ƙwayoyin cuta na cutar zawo a cikin ɗan adam, galibi suna shafar jarirai da yara ƙanana (ƙasa da shekaru 4).Lokacin shiryawa shine kusan kwanaki 3 zuwa 10.Yana kwafi a cikin ƙwayoyin hanji kuma yana fitar da shi a cikin najasa har tsawon kwanaki 10.Alamomin asibiti sune ciwon ciki, gudawa, najasa ruwa, tare da zazzabi da amai.
Norovirus (NoV)na dangin caliciviridae kuma yana da ɓangarorin 20-hedral tare da diamita na 27-35 nm kuma babu ambulaf.Norovirus yana daya daga cikin manyan cututtukan da ke haifar da gastroenteritis mai tsanani wanda ba na kwayan cuta ba a halin yanzu.Wannan kwayar cutar tana da saurin yaduwa kuma galibi ana kamuwa da ita ne ta gurbataccen ruwa, abinci, watsa lamba da iska mai iska da gurbatacciyar iska ke haifarwa.Norovirus ita ce babbar cuta ta biyu da ke haifar da gudawa a cikin yara, kuma yana fitowa a wuraren da mutane ke da yawa.Noroviruses an raba su zuwa nau'ikan kwayoyin halitta guda biyar (GI, GII, GIII, GIV da GV), kuma manyan cututtukan da ke cutar da dan adam sune GI, GII da GIV, daga cikinsu kwayoyin halittar GII sune nau'in kwayar cutar da aka fi sani a duniya.Hanyoyin bincike na asibiti ko dakin gwaje-gwaje na kamuwa da cutar norovirus galibi sun haɗa da microscopy na lantarki, ilmin kwayoyin halitta da gano rigakafi.
Abun ciki
Tarin Samfura da Gudanarwa
1. Tattara samfurin najasa bazuwar a cikin busasshiyar rumbun ajiya mai tsabta.
2. Bude na'urar tattara najasa ta hanyar kwance saman saman kuma yi amfani da felun tarin zuwa ga kauye.
3. a huda samfurin najasar a cikin shafuka daban-daban 2 ~ 5 don tattara kusan 100mg mai ƙarfi (daidai da 1/2 na fis) ko najasar ruwa 100μL.Kar a diba samfurin najasa saboda wannan na iya haifar da sakamakon gwaji mara inganci.
4. Tabbatar cewa samfurin najasa yana cikin ramukan felun tarin.Yawan samfurin najasa na iya haifar da sakamakon gwaji mara inganci.
5. Kunna kuma ƙara hular kan na'urar tattara samfuran.
6. Girgiza na'urar tattara najasa da ƙarfi.
Tsarin Gwaji
1. Kawo samfurin da gwada kayan aikin zuwa zafin daki idan an sanyaya ko daskararre.
2. Lokacin da kuka shirya don fara gwaji, buɗe jakar da aka hatimce ta yayyage tare da daraja.Cire gwajin daga jakar.
3. Sanya na'urar gwajin akan tsaftataccen wuri mai lebur.
4. Sanya na'urar tattara najasa a tsaye kuma a karkatar da hular mai rarrabawa.
5. Rike na'urar tattara najasa a tsaye, shafa 80μL (kusan digo 2) na maganin a cikin rijiyar samfurin na'urar gwaji.Kar a yi lodin samfur.
6. Karanta sakamakon gwajin a cikin mintuna 15.Kar a karanta sakamakon bayan mintuna 15.
Fassarar Sakamako
1. Mai kyau:Kasancewar layukan ja-purple biyu (T da C) a cikin taga sakamakon yana nuna tabbatacce ga RV/ADV/NoV antigen.
2. Mara kyau:Layi ja-purple ɗaya kaɗai da ke bayyana a layin sarrafawa (C) yana nuna mummunan sakamako.
3. Ba daidai ba:Idan layin sarrafawa (C) ya kasa bayyana, komai ko layin T yana bayyane ko a'a, gwajin ba ya aiki.Bita tsarin kuma maimaita gwajin tare da sabuwar na'urar gwaji.
