Kayayyaki

Cikakken Bayani:

Gwajin Innovita® 2019-nCoV IgM/IgG an yi niyya ne don bincike da kuma lura da cututtukan cututtukan da ke haifar da sabon coronavirus (2019-nCoV).ORF1ab da N gene na 2019-nCoV ana gano su da inganci daga swabs na makogwaro da samfuran ruwan lavage na alveolar da aka tattara daga waɗanda ake zargi da kamuwa da cutar huhu, marasa lafiya waɗanda ake zargi da cutar, wasu waɗanda ke buƙatar tantancewa.
Sakamakon gwajin wannan kit ɗin don nunin asibiti ne kawai.Ana ba da shawarar yin nazari mai zurfi game da yanayin dangane da bayyanar cututtuka na marasa lafiya da sauran gwaje-gwajen dakin gwaje-gwaje.

Ka'ida:

Wannan kit ɗin yana amfani da juzu'i na juzu'i na juzu'i guda ɗaya na polymerase chain reaction (RT-PCR) fasahar ganowa don yin niyya ga sabon coronavirus (2019-nCoV) ORF1ab gene, N gene, da jerin abubuwan da suka shafi cikin gida na mutum.An ƙera ƙayyadaddun ƙayyadaddun alamomi da binciken taqman a yankuna da aka adana.

Abun ciki:

Lura: 1. Batches daban-daban na reagents kada a gauraye.
2. Ba a buƙatar fitar da ingantattun sarrafawa da ingantaccen sarrafawa ba

Tsarin Gwaji:

1. Nucleic acid hakar:
Akwai na'urorin hakar RNA na kasuwanci, hakar ƙwanƙwasa maganadisu da kuma cire ginshiƙi ana ba da shawarar don wannan kit ɗin.
2. Shirye-shiryen Mix:

● Fitar da amsawar 2019-nCoV Mix A/B kuma a ajiye a dakin da zafin jiki har sai daskarewa;
● Ɗauki daidaitattun sassan (Reaction Mix A 16.5μL / T, Reaction Mix B 3.5μL / T) da kuma haɗuwa, sa'an nan kuma aliquot kowane PCR dauki tare da 20μL / tube;
● Ƙara 5μL na samfurin RNA ko iko mara kyau ko kulawa mai kyau, sa'an nan kuma rufe murfin tube;
● Sanya bututun amsawa a cikin kayan aikin PCR mai haske, kuma saita iko mara kyau / tabbatacce da sigogin samfur don amsawar RT-PCR bisa ga umarnin aiki na kayan aiki.
● Yi rikodin odar jeri na samfur

3.RT-PCR ladabi:

Saitunan da aka Shawarta: