Kayayyaki

Cikakken Bayani:

Gwajin Innovita® 2019-nCoV IgM/IgG an yi niyya ne don gano ƙarancin ƙima na rigakafin ƙwayoyin cuta zuwa sabon coronavirus (2019-nCoV) a cikin ƙwayar ɗan adam, plasma ko duka samfuran jini.
2019-nCoV ya ƙunshi manyan sunadaran tsari guda huɗu: furotin S, furotin E, furotin M da furotin N.Yankin RBD na furotin S na iya ɗaure ga mai karɓar saman sel ACE2.Neutralizing antibody yana nufin ikon daure tare da pathogen, sa'an nan kuma toshe pathogen don mamaye jiki don haifar da kamuwa da cuta.Ana iya amfani da gano maganin antibody don tantance hasashen kamuwa da cuta.

Ka'ida:

Kit ɗin shine gwajin gasa na immunochromatography na colloid don gano ƙwayoyin rigakafin ƙwayoyin cuta zuwa 2019-nCoV a cikin ƙwayar ɗan adam, plasma ko duka samfuran jini.Bayan an yi amfani da samfurin ga samfurin da kyau, idan antibodies neutralizing suna nan a cikin samfurin, antibodies neutralizing zai amsa tare da colloidal zinariya mai lakabin RBD antigen don samar da hadaddun rigakafi, kuma za a rufe wurin neutralizing na RBD antigen.Sa'an nan kuma hadaddun rigakafi da kuma alamar RBD antigen ba tare da ɗaure don neutralizing antibody yi hijira tare da nitrocellulose membrane.Lokacin da suka isa yankin gwaji (Layin T), antigen da aka yiwa lakabin RBD ba tare da ɗaure don kawar da ƙwayoyin rigakafi ba za su amsa tare da ACE2 antigen da aka lulluɓe akan membrane na nitrocellulose kuma su samar da layin shunayya-ja.Lokacin da ƙaddamar da ƙwayoyin rigakafin ƙwayoyin cuta ya fi mafi ƙanƙanci iyakar ganowa, layin ja-ja-jaya ya fi sauƙi fiye da layin sarrafawa (layin C) ko kuma babu layin ja-ja-jaya da aka kafa, sakamakon yana da kyau.Lokacin da ƙaddamar da ƙwayoyin rigakafi ya yi ƙasa da mafi ƙarancin ganowa ko kuma babu ƙwayoyin rigakafi a cikin samfurin, layin ja-ja-ja ya fi duhu fiye da layin sarrafawa, sakamakon ya zama mara kyau.
Ko da ko samfurin ya ƙunshi ƙwayoyin rigakafi na 2019-nCoV, lokacin da colloidal zinariya mai lakabin kaji IgY antibody yayi ƙaura zuwa layin sarrafawa (layin C), goat anti-chicken IgY antibody wanda aka riga aka yi akan layin sarrafawa (C) zai kama shi. layi), an kafa layin shunayya-ja.Ana amfani da layin sarrafawa (layin C) azaman kulawar tsari.Layukan sarrafawa yakamata su bayyana koyaushe a cikin windows sakamakon idan an aiwatar da aikin gwajin da kyau kuma masu sakewa suna aiki kamar yadda aka yi niyya.

Abun ciki:

Tsarin Gwaji:

1. Buɗe jakar foil na aluminum kuma fitar da kaset ɗin gwaji.
2. Aiwatar da 40μL na samfurin jini/plasma ko 60μL gabaɗayan samfurin jini zuwa samfurin da kyau.
3. Aiwatar da 40μL (2 saukad da) samfurin diluent zuwa samfurin da kyau.
4. Sanya shi a dakin da zafin jiki (15 ℃ ~ 30 ℃) na minti 15-20, kuma karanta sakamakon.

Fassarar Sakamako:

1. Kyakkyawan: Lokacin da launi na layin T ya fi nauyi fiye da na layin C ko kuma lokacin da babu layin T, yana nuna tabbatacce don neutralizing antibodies.
2. Korau: Lokacin da launi na layin T ya fi duhu ko daidai da na layin C, yana nuna mummunan don neutralizing antibodies.
3. Invalid: Lokacin da layin C ya kasa bayyana, komai layin T yana bayyane ko a'a, gwajin ba ya aiki.Maimaita gwajin tare da sabon gwaji.